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KMID : 0380020040190040315
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 4 p.315 ~ p.320
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Isolation and Cultural Characteristics of Styrene Dimer [Endocrine Disrupter] Biodegrading Microorganism
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Oh Kyung-Taek
Ryu In-Jae
Kang Chang-Min
Chung Seon-Yong
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Abstract
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We examined the culture conditions and degrading characteristics of styrene dimer (endocrine disrupter) using microorganism. The isolated microbe were consisted of 3 kinds of strain. The strains were identified to Pseudomonas sp. and Klebsiella pneumoniae by API 20E kit, but one was not identified. Single strain was not grown on the C-medium containing styrene dimer. However the complex strain YH3 could grow and we confirmed it by the broth color and O.D (optical density 660 nm). The optimal culture conditions of complex strain YH3 were 35, 1,000 ppm (v/v) of styrene dimer and pH 7.0, respectively. In tolerance test against the organic solvents, the complex strain YH3 could grow above log P=3.1, and could degrade ethyl benzene and 2,4-D, one kind of herbicide. As a result of TLC (Thin Layer Chromatography) analysis, we confirmed that the metabolite of styrene dimer was created by YH3 after 5th day, but not at control samples.
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KEYWORD
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Styrene dimer, Endocrine disrupter, biodegradability, Iog P, TLC analysis
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